Description
Pentafluoro reversed-phase column for unique selectivity
The PRP-h5 derives its hydrophobic functionality from a pentafluorinated polymer bead, delivering a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Unlike silica-based C8 or C18 columns, PRP-h5 has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than silica-based C8 or C18 columns.
PRP-h5 Overview
Pentafluoro reversed-phase column for unique selectivity The PRP-h5 utilizes the PRP-3 as its base with a pentafluorinated modification, making it more hydrophobic in nature. The PRP-h5, with its functionality derived from a pentafluorinated polymer bead, delivers a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Applications:
Organic compounds: Organic compounds: macromolecules (> 2,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics
Polymer Packings
Hamilton Polymers - Poly(styrene divinylbenzene) or PS-DVB is a robust polymer material used as the base framework for all the Hamilton polymer HPLC packing materials. The high degree of cross-linking with divinylbenzene makes the polymer bead extremely durable for high pressure applications.
Polymer columns can handle pressures over 5,000 psi, have a usable pH range of 1?13, and can be cleaned with 1.0 M sodium hydroxide to remove strongly retained material. Swelling in organic solvents such as THF or chloroform is negligible because of the cross?linking. PS-DVB resins can be operated at much higher temperatures as compared to silica based materials, and they provide excellent sample recoveries due to the lack of acidic silanol groups. Polymer columns are similar in retention characteristics to a C18, however, selectivity can be slightly different in some cases.