Superior Sample Recovery
PRP-1 columns have inherent reversed-phase separation qualities that eliminate the requirement for a stationary phase coating. Hydrolysis of the stationary phase coating is often the limiting factor in column lifetime, so the lack of coating on the PRP-1 resin extends the longevity of the column. PRP-1 also have exceptional sample recovery (? 95%).
Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample.
Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.
Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides
Hamilton Polymers - Poly(styrene divinylbenzene) or PS-DVB is a robust polymer material used as the base framework for all the Hamilton polymer HPLC packing materials. The high degree of cross-linking with divinylbenzene makes the polymer bead extremely durable for high pressure applications.
Polymer columns can handle pressures over 5,000 psi, have a usable pH range of 1?13, and can be cleaned with 1.0 M sodium hydroxide to remove strongly retained material. Swelling in organic solvents such as THF or chloroform is negligible because of the cross?linking. PS-DVB resins can be operated at much higher temperatures as compared to silica based materials, and they provide excellent sample recoveries due to the lack of acidic silanol groups. Polymer columns are similar in retention characteristics to a C18, however, selectivity can be slightly different in some cases.
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